Recent publications have shown the utility of the Myriad RBM TruCulture whole blood collection and culture system paired with nCounter gene expression analysis to profile human immune responses to immunostimulatory agents including Toll-like receptor (TLR) agonists and microorganisms. The recent application note “Human Immune Response with Myriad RBM’s TruCulture® System and the NanoString® nCounter Autoimmune Profiling Gene Expression Panel” describes an easy to use, reproducible workflow for studying immune responses from whole blood. The results showed excellent technical reproducibility and correlation between levels of expressed mRNA and protein. With minimal hands-on time, whole blood can be collected, treated, and processed to identify immune signatures for different stimuli.

One awardee will be granted 36 TruCulture tubes and TruCulture accessories and 36 samples worth of analysis with the Myriad RBM OptiMAP and the NanoString Autoimmune Profiling Panels.

Program Details

Proposals must involve gene expression profiling of whole blood samples derived from research projects in autoimmunity. One awardee will be granted:

  1. 36 TruCulture tubes of their choice (to be chosen from the following):
    • CD3/CD28 (Myriad RBM Part No. 782-001125)
    • LPS (Myriad RBM Part No. 782-001087)
    • SEB (Myriad RBM Part No. 782-001124)
    • NULL (Myriad RBM Part No. 782-001086)
  2. 36 samples for analysis using the OptiMAP panel at Myriad RBM. The OptiMAP Panel consists of 13 analytes that spans the major immune responses (ENA-78, IL-8, IFN-γ, IL-2, IL-17, IL-13, IL-12p70, IL-10, IL-23, GM-CSF, TNF-α, IL-6, and IL-1β).
  3. 36 samples worth of the NanoString nCounter Autoimmune Profiling panel. The nCounter Autoimmune Profiling Panel is a comprehensive 770 gene multiplex panel that allows you to evaluate the pathways, processes and cell types involved in autoimmune disease, chronic inflammatory disease, and aberrant immune responses.

The awardee can run their samples either through an nCounter® Analysis System provided by their institution or through a service provider. For OptiMAP analysis, the awardee will submit the TruCulture supernatants to Myriad RBM. Myriad RBM will provide the TruCulture tubes and the associated accessories (Heatblock NOT included). NanoString will provide the reagents and a data analysis service to analyze the NanoString output from the experiment in the form of a report that includes raw data and a detailed analysis with figures and commentary.

For more information on the combined TruCulture-NanoString workflow check out our quick reference guide, the Myriad RBM TruCulture website, instructional video, and manual or watch our recent joint webinar.

Grant submissions must be received by 11:59 PM Pacific Time on June 21st, 2019. The winner will be announced on or before Friday July 12th, 2019.

Publication of Results

All grant submissions are confidential to the individual submitter and will not be published or distributed. Upon project completion, each grant winner agrees to participate in one of the following activities relating to the specific project, with specific details of the applicable activity to be mutually agreed upon in good faith by NanoString/Myriad RBM and each winner: local seminar, webinar, case study, blog post, or poster presentation. Each winner agrees that NanoString/Myriad RBM shall have the right to use anonymized data from the winner’s project in application notes and/or marketing literature.

Eligibility

Applicants must be actively enrolled at an academic institution or employed at a US research facility in a capacity in which they are able to perform research on and have capabilities to collect whole blood from human subjects and have the ability to submit TruCulture supernatants to Myriad RBM for OptiMAP analysis and run the NanoString gene expression panels by December 31st, 2019.

For Research Use Only. Not for use in diagnostic procedures

Apply now through Friday June 21st!