TruCulture has been utilized as a whole-blood stimulation system by researchers and drug developers in several fields to reliably measure immune response for the following applications.

• Pharmacodynamics (including dose response)
• Functional immune cell analysis
• Disease characterization
• Patient stratification
• Genotype – to – Phenotype association studies

In one pertinent example, our partner, The Institut Pasteur, demonstrated the indispensable benefits of TruCulture in The Milieu Intérieur (“Environment Within”) project. Briefly, the Milieu Intérieur project is working to establish the determinants of a healthy immune response by identifying the genetic, epigenetic and environmental factors that contribute to the observed heterogeneity of immune responses. The challenge of the project is to establish the boundaries of a healthy immune response, and to that end, the Milieu Intérieur Project recruited 1,000 healthy individuals stratified across age (five-decades of life from age 20 – 69), and gender (1:1 gender ratio). TruCulture tubes were an integral part of the Milieu Intérieur clinical study, used as a standardized method for monitoring human response to immune stimulation.

To define the boundaries of a healthy immune response, The Milieu Intérieur project combined the use of TruCulture whole blood culture system with the use of stimuli to define the protein signatures induced by (i) medically relevant bacteria, fungi and viruses; (ii) agonists specific for defined host sensors; (iii) clinically employed cytokines; and (iv) activators of T cell immunity.

Visit The Milieu Intérieur website for more information on the project, publications, protocols, and data exploration.

View Peer-Reviewed Publications Citing TruCulture

Bibliography

TruCulture

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If the stimulant you’d like to use in our TruCulture tubes is not listed, please fill out this form to request it. One of our experts will contact you to discuss the feasibility of your request. Thank you for your interest in TruCulture.

Myriad RBM provides quantitative measurement of immune related proteins from TruCulture supernatants. Whether your specific application requires a broad range or a focused set of analytes, Myriad RBM’s panels are designed to meet your needs.

TruCulture® OptiMAP is an immunoassay testing panel validated and optimized to quantify the effect of TruCulture stimulations.  It is a cost-effective test comprised of 13 biomarkers that captures the diversity of responses to microbial, viral, and T cell stimulants.

OptiMAP Biomarkers

1. Epithelial-Derived Neutrophil-Activating Protein 78	2. Granulocyte-Macrophage Colony-Stimulating Factor
3. Interferon gamma	4. Interleukin-1 beta
5. Interleukin-10	6. Interleukin-12 Subunit p70
7. Interleukin-13	8. Interleukin-17
9. Interleukin-2	10. Interleukin-23
11. Interleukin-6	12. Interleukin-8
13. Tumor Necrosis Factor alpha

MAP	Volume Required
OptiMAP v. 1.0	TruCulture Supernatant 100 μL

OptiMAP Design

A total of 106 immune-related biomarkers were tested on TruCulture samples taken from healthy subjects. A careful selection of the key stimulants listed below were used to monitor a wide range of immune response pathways.  From the 106 candidate biomarkers, we selected a subset of 13 assays that were useful in distinguishing responses to individual stimuli while also revealing population variances.

Microbial stimulants: LPS, IL-1β, TNF-α, BCG, HK C. albicans, HK E. coli, HK S. aureus

Viral stimulants: Poly IC, R848, IFN- α/β

T cell stimulants:  SEB, anti-CD3/CD28

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For a more comprehensive understanding of functional immune cell activity, TruCulture supernatant can be tested on any of our Human MAP products. The tubes are also compatible with flow cytometry and gene expression analysis.

Multiplex immunoassay analysis of TruCulture supernatants is conducted in our CLIA-certified testing laboratory.