A Whole Blood Collection and Culture Tube for Standardized Immunophenotyping Procedures
TruCulture tubes are designed to capture immune cell activity at the time and place of sample collection, thereby minimizing the bias and variability introduced by sample shipping and manipulation. These revolutionary tubes consist of an integrated whole-blood collection and leukocyte culture system that is reliable, simple to use and does not require specialized laboratory equipment. TruCulture enables researchers to reproducibly ascertain information about immune responses to stress or stimulation, including responses to novel therapeutics. The ex vivo TruCulture procedure preserves physiological cellular interactions to more accurately reflect the complexities of the human immune system, bringing added value to immune monitoring in clinical trials.
TruCulture tubes are pre-loaded with cell culture media and your choice of stimulant. The tubes are sent frozen and shipped directly to the collection site.
1mL of blood is drawn directly into the tube.
The tubes are incubated in dry heat blocks at 37°C for at least 30 minutes and up to 48 hours, depending on the application.
After incubation, an easy to use mechanical separator is inserted to stop the reaction.
The frozen tubes should be sent to Myriad RBM’s testing laboratory for analysis of secreted cytokines and chemokines.
The tubes are also compatible with flow cytometry and gene expression analysis.
TruCulture Tube Components
TruCulture has been utilized as a whole-blood stimulation system by researchers and drug developers in several fields to reliably measure immune response for the following applications.
- Pharmacodynamics (including dose response)
- Functional immune cell analysis
- Disease characterization
- Patient stratification
- Genotype – to – Phenotype association studies
In one pertinent example, our partner, The Institut Pasteur, demonstrated the indispensable benefits of TruCulture in The Milieu Intérieur (“Environment Within”) project. Briefly, the Milieu Intérieur project is working to establish the determinants of a healthy immune response by identifying the genetic, epigenetic and environmental factors that contribute to the observed heterogeneity of immune responses. The challenge of the project is to establish the boundaries of a healthy immune response, and to that end, the Milieu Intérieur Project recruited 1,000 healthy individuals stratified across age (five-decades of life from age 20 – 69), and gender (1:1 gender ratio). TruCulture tubes were an integral part of the Milieu Intérieur clinical study, used as a standardized method for monitoring human response to immune stimulation.
To define the boundaries of a healthy immune response, The Milieu Intérieur project combined the use of TruCulture whole blood culture system with the use of stimuli to define the protein signatures induced by (i) medically relevant bacteria, fungi and viruses; (ii) agonists specific for defined host sensors; (iii) clinically employed cytokines; and (iv) activators of T cell immunity.
View an Overview of The Milieu Intérieur Project:
View Peer-Reviewed Publications Citing TruCulture
TruCultureThere are no Bibliographies for this Product
Milieu Intérieur: Personalized Immune Response Monitoring
“We hope to advance the promise of personalized medicine with functional biomarkers.”
Dr. Matthew Albert, Director of the Department of Immunology; Co-Director and Founder of the Center for Human Immunology at Institut Pasteur; Adjunct Faculty at Cochin Hospital
Applications of TruCulture in Chronic infection
“What makes our project unique is our ability to induce standardized immune responses…possible because of the reproducibility of the TruCulture system.”
Dr. Darragh Duffy, Laboratory of Dendritic Cell Biology, Department of Immunology, Institut Pasteur
Statistical Analysis and Mathematical Modeling of Data Derived from TruCulture
“[Using TruCulture] we get high quality data with very little variation.”
Pr. Magnus Fontes, Director of the International Group for Data Analysis, Institut Pasteur
|782-001086||Null TruCulture Tubes (TC Tubes Null)||Control|
|782-001219||TNF-alpha (TC Tubes TNFa 10ng/ml)||Cytokine|
|782-001224||Intron A, IFN-alpha (TC Tubes IFNa)||Cytokine|
|782-001277||Interferon beta (TC Tubes IFN-beta)||Cytokine|
|782-001278||Interleukin-1beta + tumor necrosis factor-alpha (TC Tubes IL-1b+TNFa)||Cytokine|
|782-001279||Intron A + anti-CD3 + anti-CD28 (TC Tubes IFNa+CD3+CD28)||Cytokine|
|782-001225||Intron A + LPS-EB high (TC Tubes IFNa+LPS-EB)||Cytokine / TLR4 Ligand|
|782-001272||Adenosine Triphosphate + Lipopolysaccharide, high concentration (TC Tubes ATP+LPS-EB)||NLRP3 Inflammasome / TLR4 Ligand|
|782-001273||Lauroyl-γ-D-glutamyl-meso-diaminopimelic acid (TC Tubes C12-iE-DAP)||NOD Ligand|
|782-001280||Lipoarabinomannan from M. smegmatis (TC Tube LAM-MS)||NOD Ligand|
|782-001124||SEB TruCulture Tubes (TC Tubes SEB)||T-Cell|
|782-001125||CD3/CD28 TruCulture Tubes (TC Tubes CD3+CD28)||T-Cell|
|782-001126||SEB/CD28 TruCulture Tubes (TC Tubes SEB+CD28)||T-Cell|
|782-001202||CD3 TruCulture Tubes (TC Tubes CD3)||T-Cell|
|782-001259||Zymosan TruCulture Tubes (TC Tubes ZYM)||TLR2 Ligand|
|782-001274||Fibroblast-stimulating Lipopeptide (TC Tubes FSL-1)||TLR2 Ligand|
|782-001275||HKEB (TC Tubes HKEB)||TLR2 Ligand|
|782-001276||HKLR (TC Tubes HKLR)||TLR2 Ligand|
|782-001282||Polyinosinic:polycytidylic acid (TC Tube Poly I:C)||TLR3 Ligand|
|782-001087||LPS TruCulture Tubes (TC Tubes LPS)||TLR4 Ligand|
|782-001089||LPS+SEB TruCulture Tubes (TC Tubes LPS+SEB)||TLR4 Ligand / T-Cell|
|782-001264||Resiquimod R848 (TC Tubes R848)||TLR7/8 Ligand|
|782-001269||Gardiquimod (TC Tubes GDQ)||TLR7/8 Ligand|
|782-001281||Class A CpG oligonucleotide + LPS-EB high (TC Tube ODN2216+LPS-EB)||TLR9 Ligand|
If the stimulant you’d like to use in our TruCulture tubes is not listed, please fill out this form to request it. One of our experts will contact you to discuss the feasibility of your request. Thank you for your interest in TruCulture.
Myriad RBM provides quantitative measurement of immune related proteins from TruCulture supernatants. Whether your specific application requires a broad range or a focused set of analytes, Myriad RBM’s panels are designed to meet your needs.
TruCulture® OptiMAP is an immunoassay testing panel validated and optimized to quantify the effect of TruCulture stimulations. It is a cost-effective test comprised of 13 biomarkers that captures the diversity of responses to microbial, viral, and T cell stimulants.
|OptiMAP v. 1.0||TruCulture Supernatant 100 μL|
A total of 106 immune-related biomarkers were tested on TruCulture samples taken from healthy subjects. A careful selection of the key stimulants listed below were used to monitor a wide range of immune response pathways. From the 106 candidate biomarkers, we selected a subset of 13 assays that were useful in distinguishing responses to individual stimuli while also revealing population variances.
Microbial stimulants: LPS, IL-1β, TNF-α, BCG, HK C. albicans, HK E. coli, HK S. aureus
Viral stimulants: Poly IC, R848, IFN- α/β
T cell stimulants: SEB, anti-CD3/CD28
For a more comprehensive understanding of functional immune cell activity, TruCulture supernatant can be tested on any of our Human MAP products. The tubes are also compatible with flow cytometry and gene expression analysis.
Multiplex immunoassay analysis of TruCulture supernatants is conducted in our CLIA-certified testing laboratory.
Co-Developing new tubes
To co-develop Truculture tubes of interest, please contact us.
Myriad RBM can evaluate your stimuli of interest for sterility, solubility, dose response, short and long-term stability, and reproducibility.
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