Rules-Based Medicine (RBM) is a CLIA certified laboratory providing researchers with reproducible, quantitative immunoassay data from a single drop of blood serum or plasma.
We develop and manufacture our own assays in order to control for lot-to-lot variability and minimize matrix interferences (RF factor, heterophile antibodies).
Measure 55 key inflammation markers in a cost-effective, and efficient manner from 5 validated multiplex panels covering inflammatory pathways including cytokines, chemokines, vascular proteins and acute-phase reactants. Our biomarker testing services have been used in hundreds of clinical trials and research projects and cited in over 1000 peer reviewed publications.
Blue Text = Multiplex Immunoassays | = Ultrasensitive Immunoassays
- GM-CSF-based treatments in COVID-19: reconciling opposing therapeutic approaches
(2020) Lang FM, et al. Nature Reviews Immunology. 20, 507–514 (2020)
- Impaired type I interferon activity and inflammatory responses in severe COVID-19 patients.
Hadjadj J, et al. Science 369(6504):718-724.v
Recent coverage of the Hadjadj J, et al. article in BioSpace
- Type III interferons disrupt the lung epithelial barrier upon viral recognition.
(2020) Broggi A, et al. Science 369(6504):706-712
- Type I and III interferons disrupt lung epithelial repair during recovery from viral infection
Major J, et al. Science 369(6504):712-717
- Cerecor and Myriad Genetics announce that levels of novel cytokine, LIGHT were highly correlated with disease severity and mortality in COVID-19 ARDS biomarker study
Harrell J and Gleason S. (2020) https://myriadrbm.com/2020/05/26/cerecor-and-myriad-genetics-announce-that-levels-of-novel-cytokine-light-were-highly-correlated-with-disease-severity-and-mortality-in-covid-19-ards-biomarker-study/
- Neuropilin-1 is a host factor for SARS-CoV2 infection
(2020) Daly JL, et al. Science.
- Neuropilin-1 facilitates SARS-CoV2 cell entry and infectivity. (2020) Cantuti-Castelvetri L, et al. Science
- Growth differentiation factor-15 provides prognostic information superior to established cardiovascular and inflammatory biomarkers in unselected patients hospitalized with COVID-19
(2020) Myhre PL, et al. Circulation
- The role of Interleukin 6 inhibitors in therapy of severe COVID-19
(2020) Nasanov E, et al. Biomedicine & Pharmacotherapy Volume 131, 2020
- A dynamic COVID-19 immune signature includes associations with poor prognosis
(2020) Laing AG, et al. Nature Medicine. 26, 1623–1635 (2020)
- The Inhibition of IL-2/IL-2R gives rise to CD8+T cell and lymphocyte decrease through JAK1-STAT5 in critical patients with COVID-19 pneumonia
(2020) Shi H, et al. Cell Death Dis. 2020 Jun 8;11(6):429.
- Biomarkers of COVID-19 and technologies to combat SARS-CoV2
(2020) Zhang L and Guo H. Advances in Biomarker Sciences and Technology. 2020;2:1-23
- Longitudinal immune profiling reveals key myeloid signatures associated with COVID-19
(2020) Mann ER, et al. Science Immunology 5,5. eabd6197
- Immunophenotyping of COVID-19 and influenza highlights the role of type I interferons in development of severe COVID-19
(2020) Lee LS, et al. Science Immunology. 5, 49, eabd1554
- COVID-19: consider cytokine syndromes and immunosuppression
(2020) Mehta P, et al. The Lancet. 395, 10229. 1033-1034
- Serum IgA, IgM, and IgG response in COVID-19
(2020) Ma H, et al. Cellular & Molecular Immunology. 17, 773-775
- Matrix metalloproteinase 3 as a valuable marker for patients with COVID-19
(2020) Shi S, et al. J Med Virol. 10.1002/jmv.26235
- Inhibition of cytokine signaling by ruxolitinib and implications for COVID-19 treatment
(2020) Yeleswarm S, et al. Clinical Immunology. 218, 108517
- Interferon gamma, TGF-b1 and RANTES expression in upper airway samples from SARS-CoV2 infected patients
(2020) Clinical Immunology. 220, 108576.
TruCulture is a whole blood collection and culture system for immune monitoring in subjects and has been utilized in hundreds of clinical trials. The SARS-CoV2 Spike Protein TruCulture tube was designed and developed to be used in clinical trials to:
- Quantify SARS-CoV2 vaccine-dependent T cell activation.
- Investigate the magnitude and duration of T cell responses post SARS-CoV2 infection.
Use TruCulture tubes to collect 1 mL of whole blood directly from a patient to immediately start antigen recognition, processing, and presentation in the tube. Each TruCulture tube contains 2 mL of media including the SARS-CoV2 spike protein antigen. After 48 hours at 37o C, the secreted T cell cytokines IFNγ and IL-2 are quantified by validated immunoassays (Myriad RBM’s ultrasensitive immunoassay services)
Download the SARS-CoV2 Spike Protein TruCulture Tubes application note to learn more. ➡
For other TruCulture Tubes and Stimulants, click here.
Draw 1 mL of blood directly into the TruCulture Tube and break off the plunger.
Gently invert tube to mix 3 to 5 times
Place tube in 37ºC heat block for up to 24 or 48 hours.
Manually insert valve to separate supernatant from the cells. Collect supernatant and cell layer for downstream analysis.
By submitting your information in this form, you agree that your personal information may be stored and processed in any country where we have facilities or service providers, and by using our “Contact Us” page you agree to the transfer of information to countries outside of your country of residence, including to the United States, which may provide for different data protection rules than in your country. The information you submit will be governed by our Privacy Notice.
The RBM MAP platform has been built and optimized to help researchers discover and validate biomarker patterns for use in their drug and diagnostic development efforts. Robust multiplexing on the microsphere-based Luminex technology combined with automated liquid handling, delivers more data in less time than other platforms, resulting in comparable data to traditional ELISA assays.
- High throughput multiplexing with automated liquid handling reduces sample volume requirements
- Enhanced accuracy with use of multi-level controls, standard curves, and proprietary blockers
- High quality and reproducibility with stringent quality control parameters for reliable data
We ensure quality for every multiplex, every analyte, every sample, every time.
The RBM platform produces precise and dependable results by utilizing automated liquid handling systems, advanced quality monitoring, validated data reporting processes, and a highly-trained and dedicated staff.
Every analyte included in RBM’s Multi-Analyte Profiles (MAPs) has its own set of calibrators and controls to provide reliable, high quality data. By continuous quality improvement, RBM ensures that customers are satisfied with the products and services they receive.
Our CustomMAP is a flexible option to let you pick specific biomarkers for your research needs. Customize the number of multiplexes to build your CustomMAP.
Speak to a sales representative to learn more about CustomMAP options.
50 sample minimum required. Volume requirements are dependent on the number of multiplexes selected.
Utilized in hundreds of clinical trials for a variety of applications, TruCulture® is a whole blood collection and culture system for immune monitoring of subjects in clinical trials.
- Detect vaccine-dependent T-cell activation (antigen recall)
- Early safety studies of vaccines and their individual components
- Profile a subject’s functional immune status