Cytokine Storm Testing

Immune Monitoring Solutions for COVID-19 Research

Cytokine Storm Testing for SARS-CoV-2 & COVID-19 Research

Myriad RBM is a CLIA certified laboratory providing researchers with reproducible, quantitative immunoassay data from a single drop of blood serum or plasma.

We develop and manufacture our own assays in order to control for lot-to-lot variability and minimize matrix interferences (RF factor, heterophile antibodies).

InflammationMAP® and Immune Markers for COVID-19

Measure 55 key inflammation markers in a cost-effective, and efficient manner from 5 validated multiplex panels covering inflammatory pathways including cytokines, chemokines, vascular proteins and acute-phase reactants. Our biomarker testing services have been used in hundreds of clinical trials and research projects and cited in over 1000 peer reviewed publications.

InflammationMAP®

CORE1 (CytokineMAP®A)
1. GM-CSF 1, 12, 18
2. IFN-gamma 2, 3, 4, 18, 19
3. IL-2 11, 15, 18
4. IL-3
5. IL-4 18
6. IL-5
7. IL-6 9, 10, 12, 13, 18
8. IL-7 15, 18
9. IL-8 12, 18
10. IL-10 10, 12, 18
11. IL-18
12. MIP-1 alpha 15, 18
13. MIP-1 beta 18
14. MCP-1 13, 18
15. TNF-alpha 12, 14, 15, 18
16. TNF-beta 12, 14
Core2 (CytokineMAP®B)
1. BDNF
2. Eotaxin-1
3. Factor VII
4. ICAM-1
5. IL-1 alpha
6. IL-1 beta 14, 17, 18
7. IL-1ra
8. IL-12p40
9. IL-12p70
10. IL-17 18
11. IL-23
12. MMP-3 17
13. MMP-9
14. SCF
15. VEGF 18
HMP8
1. Adiponectin
2. A2Macro
3. EN-RAGE
4. Ferritin 15
5. Myoglobin
6. PAI-1
7. PARC
8. RANTES 19
9. TIMP-1
10. TNFR2
11. VCAM-1
HMP2
1. Lp(a)
2. B2M
3. CRP 12
4. SAP
5. TBG
6. vWF
Core4
1. AAT
2. C3
3. Fibrinogen
4. Haptoglobin
5. Ig A 16
6. Ig M 16
7. VDBP

Additional Markers

1. Interferon alpha 2, 3, 4
2. Interferon beta 2, 3, 4  Ultrasensitive Immunoassay
3. Neurofilament Light Chain 5, 12  Ultrasensitive Immunoassay
4. Neuropilin-1 6, 7
5. Growth/differentiation factor 15 8
6. Interferon gamma Induced Protein 10 10, 13, 18
7. Interleukin-2 receptor alpha 11
8. LAP-TGF-beta 19

References

  1. GM-CSF-based treatments in COVID-19: reconciling opposing therapeutic approaches
    (2020) Lang FM, et al. Nature Reviews Immunology. 20, 507–514 (2020)
    https://doi.org/10.1038/s41577-020-0357-7
  2. Impaired type I interferon activity and inflammatory responses in severe COVID-19 patients.
    Hadjadj J, et al. Science 369(6504):718-724.v
    Recent coverage of the Hadjadj J, et al. article in BioSpace
  3. Type III interferons disrupt the lung epithelial barrier upon viral recognition.
    (2020) Broggi A, et al. Science 369(6504):706-712
  4. Type I and III interferons disrupt lung epithelial repair during recovery from viral infection
     Major J, et al. Science 369(6504):712-717
  5. Cerecor and Myriad Genetics announce that levels of novel cytokine, LIGHT were highly correlated with disease severity and mortality in COVID-19 ARDS biomarker study
    Harrell J and Gleason S. (2020) https://myriadrbm.com/2020/05/26/cerecor-and-myriad-genetics-announce-that-levels-of-novel-cytokine-light-were-highly-correlated-with-disease-severity-and-mortality-in-covid-19-ards-biomarker-study/
  6. Neuropilin-1 is a host factor for SARS-CoV-2 infection
    (2020) Daly JL, et al. Science.
    DOI:10.1126/science.abd307
  7. Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity. (2020) Cantuti-Castelvetri L, et al. Science
    DOI: 10.1126/science.abd2985
  8. Growth differentiation factor-15 provides prognostic information superior to established cardiovascular and inflammatory biomarkers in unselected patients hospitalized with COVID-19
    (2020) Myhre PL, et al. Circulation 
    DOI: 10.1161/CIRCULATIONAHA.120.050360
  9. The role of Interleukin 6 inhibitors in therapy of severe COVID-19
    (2020) Nasanov E, et al. Biomedicine & Pharmacotherapy Volume 131, 2020
    https://doi.org/10.1016/j.biopha.2020.110698
  10. A dynamic COVID-19 immune signature includes associations with poor prognosis
    (2020) Laing AG, et al. Nature Medicine. 26, 1623–1635 (2020)
    https://doi.org/10.1038/s41591-020-1038-6
  11. The Inhibition of IL-2/IL-2R gives rise to CD8+T cell and lymphocyte decrease through JAK1-STAT5 in critical patients with COVID-19 pneumonia
    (2020) Shi H, et al. Cell Death Dis. 2020 Jun 8;11(6):429.
    DOI: 10.1038/s41419-020-2636-4
  12. Biomarkers of COVID-19 and technologies to combat SARS-CoV-2
    (2020) Zhang L and Guo H. Advances in Biomarker Sciences and Technology. 2020;2:1-23
    doi: 10.1016/j.abst.2020.08.0011,
  13. Longitudinal immune profiling reveals key myeloid signatures associated with COVID-19
    (2020) Mann ER, et al. Science Immunology 5,5. eabd6197
    DOI:10.1126/sciimmunol.abd6197
  14. Immunophenotyping of COVID-19 and influenza highlights the role of type I interferons in development of severe COVID-19
    (2020) Lee LS, et al. Science Immunology. 5, 49, eabd1554
    DOI:10.1126/sciimmunol.abd1554
  15. COVID-19: consider cytokine syndromes and immunosuppression
    (2020) Mehta P, et al. The Lancet. 395, 10229. 1033-1034
    https://doi.org/10.1016/S0140-6736(20)30628-0
  16. Serum IgA, IgM, and IgG response in COVID-19
    (2020) Ma H, et al. Cellular & Molecular Immunology. 17, 773-775
    https://doi.org/10.1038/s41423-020-0474-z
  17. Matrix metalloproteinase 3 as a valuable marker for patients with COVID-19
    (2020) Shi S, et al. J Med Virol. 10.1002/jmv.26235
    DOI: 10.1002/jmv.26235
  18. Inhibition of cytokine signaling by ruxolitinib and implications for COVID-19 treatment
    (2020) Yeleswarm S, et al. Clinical Immunology. 218, 108517
    https://doi.org/10.1016/j.clim.2020.108517
  19. Interferon gamma, TGF-b1 and RANTES expression in upper airway samples from SARS-CoV-2 infected patients
    (2020) Clinical Immunology. 220, 108576.
    https://doi.org/10.1016/j.clim.2020.108576

Coming Soon!

CoV SerologyMAP

CoV SerologyMAP™ — A 17-plex coronavirus antigen IgG serology assay

Take a deep dive into the humoral immune response to SARS-CoV-2 and related coronaviruses.

>99% sensitivity and specificity achieved for determining SARS-CoV-2 infection by simultaneously determining reactivity to:

  1. SARS-CoV-2 Spike trimer – antigen titer (4 different bead concentrations)
  2. S-CoV-2 Spike RBD – antigen titer (3 different bead concentrations)
  3. SARS-CoV-2 Nucleocapsid
  4. SARS-CoV-2 N-terminal domain (NTD)
  5. MERS-CoV Spike
  6. SARS-CoV-1 Spike
  7. HCoV-HKU1 (common cold β-coronavirus)
  8. HCoV-OC43 (common cold β-coronavirus)
  9. HCoV-229E (common cold α-coronavirus)
  10. HCoV-NL63 (common cold α-coronavirus)
  11. BSA – Negative Control
  12. Anti-human IgG (sample addition positive control)

All this in a single well! No multi-well sample titers required, only 25 μl of sample needed.

Applications:

  1. Profile the serological response to vaccine candidates
  2. Investigate the humoral response to SARS-CoV-2 infection including Ab cross reactivity patterns to six other coronaviruses
  3. Identify false positives from large population seroconversion studies

Assay principle: CoV protein antigens are coupled to individual Luminex beads sets that are subsequently combined. Reactive antibodies (IgG) from serum or plasma (EDTA, Citrate or Heparin) samples are detected using phycoerythrin labelled anti-human IgG antibodies.

“For research Use Only”

SARS-CoV-2 T cell activation with NEW TruCulture Spike Protein tubes

SARS-CoV-2 Spike Protein TruCulture Tubes

TruCulture® is a whole blood collection and culture system for immune monitoring in subjects and has been utilized in hundreds of clinical trials. The SARS-CoV-2 Spike Protein TruCulture tube was designed and developed to be used in clinical trials to:

  1. Quantify SARS-CoV-2 vaccine-dependent T cell activation.
  2. Investigate the magnitude and duration of T cell responses post SARS-CoV-2 infection.

Use TruCulture tubes to collect 1 mL of whole blood directly from a patient to immediately start antigen recognition, processing, and presentation in the tube. Each TruCulture tube contains 2 mL of media including the SARS-CoV-2 spike protein antigen. After 48 hours at 37o C, the secreted T cell cytokines IFNγ and IL-2 are quantified by validated immunoassays (Myriad RBM’s ultrasensitive immunoassay services)

Download the SARS-CoV-2 Spike Protein TruCulture Tubes application note to learn more. ➡

For other TruCulture Tubes and Stimulants, click here.

Analysis

TruCulture cell pellets can be collected for downstream analyses:

Application Note Download

Download the SARS-CoV-2 Spike Protein TruCulture Tubes application note to learn how TruCulture tubes are used to measure antigen recall responses to the SARS-CoV-2 spike protein.

TruCulture Procedure, Collect

01. COLLECT

Draw 1 mL of blood directly into the TruCulture Tube and break off the plunger.

TruCulture Procedure, MIX

02. MIX

Gently invert tube to mix 3 to 5 times

TruCulture Procedure, Incubate

03. INCUBATE

Place tube in 37ºC heat block for up to 24 or 48 hours.

TruCulture Procedure, separate

04. SEPARATE

Manually insert valve to separate supernatant from the cells. Collect supernatant and cell layer for downstream analysis.

Watch our instructional video to see how easy it is to use TruCulture.

All you need is a phlebotomist and a 37oC heat block!
No need for specialized cell culture equipment or technical knowledge.

CONTACT US

Speak to a Scientist

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Multi-Analyte Profile (MAP) Platform

The Myriad RBM MAP platform has been built and optimized to help researchers discover and validate biomarker patterns for use in their drug and diagnostic development efforts. Robust multiplexing on the microsphere-based Luminex technology combined with automated liquid handling, delivers more data in less time than other platforms, resulting in comparable data to traditional ELISA assays.

  • High throughput multiplexing with automated liquid handling reduces sample volume requirements
  • Enhanced accuracy with use of multi-level controls, standard curves, and proprietary blockers
  • High quality and reproducibility with stringent quality control parameters for reliable data

We ensure quality for every multiplex, every analyte, every sample, every time.
The Myriad RBM platform produces precise and dependable results by utilizing automated liquid handling systems, advanced quality monitoring, validated data reporting processes, and a highly-trained and dedicated staff.

Every analyte included in Myriad RBM’s Multi-Analyte Profiles (MAPs) has its own set of calibrators and controls to provide reliable, high quality data. By continuous quality improvement, Myriad RBM ensures that customers are satisfied with the products and service they receive.

xMAP

Other Myriad RBM Products

CustomMAP Builder

Our CustomMAP is a flexible option to let you pick specific biomarkers for your research needs. Customize the number of multiplexes to build your CustomMAP.

Speak to a sales representative to learn more about CustomMAP options.

50 sample minimum required. Volume requirements are dependent on the number of multiplexes selected.

Ultrasensitive Immunoassays - Simoa

Looking for trace quantities of markers? Ultrasensitive immunoassays achieve orders-of-magnitude greater sensitivity than conventional immunoassay platforms for when levels of key proteins are extremely low in serum or plasma.

simoa

SARS-CoV-2 Vaccine Development Application with TruCulture®

Utilized in hundreds of clinical trials for a variety of applications, TruCulture® is a whole blood collection and culture system for immune monitoring of subjects in clinical trials.

  • Detect vaccine-dependent T-cell activation (antigen recall)
  • Early safety studies of vaccines and their individual components
  • Profile a subject’s functional immune status
TruCulture Vaccine Development Application note cover page