Human Sample Volume Requirements
MAPVolume Required
Serum or plasmaOther fluids*
Human DiscoveryMAP®650 µL3 mL
Human ExplorerMAP™300 µL800 µL
HumanMAP®150 µL650 µL
Human CardiovascularMAP®200 µL700 µL
Human AngiogenesisMAP®250 µL500 µL
Human InflammationMAP®50 µL300 µL
Human ImmunoMAP®200 µL650 µL
Human NeuroMAP™100 µL500 µL
Human MetabolicMAP®50 µL300 µL
Human KidneyMAP®50 µL350 µL
Human CytokineMAP® A50 µL125 µL
Human CytokineMAP® B50 µL125 µL
Vectra® DA**300 µLn/a

* Cerebrospinal fluid, urine, tissue culture supernatants,  bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.

** Serum is the only accepted sample type for Vectra DA testing. If MAP and Vectra DA testing services are requested, please provide a separate electronic sample manifest and sample aliquot for Vectra DA testing. All Vectra DA testing is performed at Crescendo Biosciences, Inc.

Simoa™ Services
BiomarkerLLOQ*
(Serum and Plasma)
LLOQ*
(Undiluted Samples)
Volume Required
Serum or plasmaOther fluids**
Amphiregulin (AR)0.24 pg/mL0.06 pg/mL100 µL150 µL
B Lymphocyte Chemoattractant (BLC, CXCL-13)0.64 pg/mL0.064 pg/mL50 µL150 µL
Granulocyte Colony-Stimulating Factor (G-CSF)0.59 pg/mL0.059 pg/mL50 µL150 µL
Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF)0.036 pg/mL0.009 pg/mL100 µL150 µL
Growth/differential factor 11 (GDF-11)5.00 pg/mL1.25 pg/mL100 µL150 µL
Interferon alpha (IFN-alpha)0.11 pg/mL0.055 pg/mL150 µL150 µL
Interferon beta (IFN-beta)0.16 pg/mL0.04 pg/mL100 µL150 µL
Interferon gamma (IFN-gamma)0.019 pg/mL0.0095 pg/mL150 µL150 µL
Interleukin-1 beta (IL-1 beta)0.031 pg/mL0.016 pg/mL150 µL150 µL
Interleukin-2 (IL-2)0.050 pg/mL0.025 pg/mL150 µL150 µL
Interleukin-5 (IL-5)0.052 pg/mL0.026 pg/mL150 µL150 µL
Interleukin-6 (IL-6)0.091 pg/mL0.0046 pg/mL50 µL150 µL
Interleukin-8 (IL-8)0.86 pg/mL0.0287 pg/mL50 µL150 µL
Interleukin-9 (IL-9)0.35 pg/mL0.0875 pg/mL100 µL150 µL
Interleukin-10 (IL-10)0.037 pg/mL0.0092 pg/mL100 µL150 µL
Interleukin-12 Subunit p40 (IL-12p40)7.30 pg/mL0.730 pg/mL50 µL150 µL
Interleukin-13 (IL-13)0.099 pg/mL0.005 pg/mL150 µL150 µL
Interleukin-17A (IL-17A)0.048 pg/mL0.012 pg/mL100 µL150 µL
Interleukin-17C (IL-17C)0.26 pg/mL0.13 pg/mL150 µL150 µL
Interleukin-31 (IL-31)0.024 pg/mL0.006 pg/mL100 µL150 µL
Kidney Injury Molecule-1 (KIM-1)3.10 pg/mL0.155 pg/mL50 µL150 µL
Oncostatin-M (OSM)0.14 pg/mL0.07 pg/mL150 µL150 µL
Oncostatin-M-Specific Receptor Subunit beta (OSMR)0.16 pg/mL0.0032 pg/mL50 µL150 µL
Receptor Activator of Nuclear Factor Kappa B Ligand (RANKL)0.58 pg/mL0.145 pg/mL100 µL150 µL
Tumor Necrosis Factor-alpha (TNF-α)0.078 pg/mL0.020 pg/mL100 µL150 µL
Tumor Necrosis Factor Ligand Superfamily Member 14 (LIGHT free)1.5 pg/mL0.0075 pg/mL50 µL150 µL
Tumor Necrosis Factor Ligand Superfamily Member 14 Total (LIGHT Total)0.56 pg/mL0.14 pg/mL100 µL150 µL
Neurofilament Light Chain (NF-L)1.00 pg/mL0.25 pg/mL120 µL200 µL

* Lower limit of quantitation (LLOQ) represents the lowest amount of an analyte that can be quantitatively determined with acceptable precision. LLOQ is determined by performing 2-fold serial dilutions of Standard to be tested in triplicate over three runs. The percent coefficient of variation (CV) is calculated for each of the dilution replicates, and the LLOQ is determined as the concentration at which the CV is 30%.

** Cerebrospinal fluid, urine, tissue culture supernatants,  bronchoalveolar lavage, synovial fluid, tissue extracts, tears, skin washings, etc.

  • Filter paper blood spots
  • Other fluid samples including:
    • Tissue culture supernatant – Caution- biotin concentrations must be less than 0.65µM (
    • Urine
    • Cerebrospinal Fluid
    • Peritoneal fluid
    • Cell lysates
    • BALF (broncheoalveolar lavage fluid)
    • Tissue homogenates – Procedure
    • Synovial fluid
  • Do not send whole blood
  • Contact a Myriad RBM Representative to confirm sample volume requirements

If your volumes are not sufficient, please contact Myriad RBM for other options and information.