Lin Wang, Carlos D. Rosé, Kevin P. Foley, Jordi Anton, Brigitte Bader-Meunier, Philippe Brissaud, Gaelle Chédeville, Rolando Cimaz, Jorge Fernández-Martin, Catherin Guly, Eric Hachulla, Miroslav Harjacek, Friederike Mackensen, Rosa Merino, Consuelo Modesto, Antonio Naranjo Hernández, Christine Pajot, Athimalaipet V. Ramanan, Akaluck Thatayatikom, Caroline Thomée, Sebastiaan Vastert, Bart J. Votta, John Bertin, and Carin H. Wouters.Rheumatology 2018 Read Full Article » Blau syndrome is an inherited auto-inflammatory disease that is characterized by arthritis, dermatitis, and uveitis. Patients also experience the presence of non-caseating granulomas, suggesting that the disease pathogenesis involves activated macrophages. The current management of Blau disease, using familiar drug classes such as corticosteroids, DMARDS and biologics, is not adequate. In order to assist in future drug development studies, biomarkers for Blau disease activity will be extremely useful. Phagocyte specific S100 proteins, such as S100A12, S100A8, and S100A9, have been proposed as biomarkers for Blau syndrome. These proteins are secreted by neutrophils and macrophages upon activation. This prospective cohort study will aim to correlate disease activity with inflammatory and S100 proteins.
Timothy Garvey, Luc Van Gaal, Lawrence A. Leiter, Ujjwala Vijapurkar, James List, Robert Cuddihy, Jimmy Ren, Michael J. DaviesMetabolism. 2018. doi: 10.1016/j.metabol.2018.02.002 Type 2 diabetic patients with increased visceral fat mass and insulin resistance are at a higher risk for cardiovascular disease and premature death. The increased visceral fat impairs adipose tissue function and is the result of combinatorial processes, including increased macrophage recruitment (MCP-1 production), increased inflammatory cytokines (IL-6 and TNF-a secretion), and reduced anti-inflammatory factors (adipokine and adiponectin).
Standardized whole blood stimulation system proves superior reproducibility over PBMCsA new paper on improved methods for monitoring differential immune response activation was published in Volume 183 of the journal Clinical Immunology. Researchers report on a multi-center study in seven FOCIS Centers of Excellence to directly compare whole blood syringe-based system, TruCulture, to the current method of isolation of PBMCs for immunomonitoring. To compare intra and inter-center variability between PBMC and TruCulture stimulation, the blood of 152 healthy donors was both stimulated in whole blood assay and after isolation of PBMC, across multiple centers. Following exposure to LPS, anti-CD3/anti-CD28, or media alone, samples were analyzed using multi-analyte profiling (MAP) for 55 proteins at Myriad RBM’s CLIA-certified laboratory. Focusing on analytes that were induced in both TruCulture and PMBC stimulation, further analysis was based on the 35 commonly induced proteins.
3D Growth of Endothelial Cells During ISS Space Mission – The SPHEROIDS Project
The April 2017 issue of Biomaterials reports on an automated cell culture unit that was developed for the SpaceX CRS-ISS space mission that resulted in three dimensional growth of human endothelial cells in microgravity.
A spaceflight to the International Space Station, carried out in April – May 2016, included a study to examine endothelial cell (EC) behavior in respect to cellular proliferation and apoptosis under spaceflight conditions to examine the gravity dependency of EC tube formation.